Postharvest of Fruit and Vegetables
New reduced-risk fungicides are highly effective in managing both pre- and postharvest diseases of stone fruit crops in California, and they have replaced most previously registered materials. These fungicides have a low impact on the environment, high specificity to target organisms, and low potential for groundwater contamination and human health risks. In stone fruit, they can be successfully used to manage brown rot blossom blight in a new delayed-bloom fungicide application program for low precipitation years. In our studies, we found that fungicide treatments applied 1 to 14 days before harvest were also effective against preharvest brown rot and protected fruit from fungal decays initiated during harvest. Practices such as mixtures and rotations can be implemented to prevent resistance from developing and to ensure the lasting efficacy of these reduced-risk fungicides.
Two approaches, fungicide applications to trees before harvest and drenching fruit after harvest, were evaluated to minimize postharvest green mold, caused by Penicillium digitatum, particularly among fruit subjected to ethylene gas after harvest, a practice termed “degreening” that eliminates green rind color. Preharvest applications of thiophanate methyl (TM) controlled postharvest green mold consistently. In five tests, green mold among degreened orange fruit was 16% when TM was applied 1 week before harvest; whereas, among fruit not treated, the incidence was 89.5%. Thiabendazole (TBZ) applied to harvested fruit in bins before degreening also was very effective. TBZ effectiveness was enhanced by mild heating (41ºC), adding sodium bicarbonate, and immersing fruit, rather than drenching them, with the solution. With these measures, an isolate of P. digitatum with a high level of TBZ resistance was significantly controlled. In semicommercial tests with naturally inoculated fruit, TBZ and sodium bicarbonate treatment reduced green mold incidence from 11% among untreated orange fruit to 2%. TBZ residues in lemon fruit at 41ºC were about twice those treated at 24ºC. Neither TM before harvest nor TBZ and sodium bicarbonate applied after harvest influenced green color removal during degreening of orange fruit. Sodium bicarbonate slightly reduced the rate of lemon color change.

The effect of pre- and postharvest applications of 200 and 400 μgL-1 aqueous solutions of 1-methylcyclopropene (1-MCP) on yellow pitahaya (maturity stage 3) was assessed. Changes in respiratory rate, color, firmness, soluble solids content, titratable acidity, maturity index, weight loss and commercial preservation were measured. Results showed that the preharvest application of 400 μgL-1 1-MCP enhanced fruit color, preserved firmness, and reduced weight loss and maturity index of fruits. It is suggested that these results were explained by the reduction of available membrane receptors able to bind ethylene, thus increasing ethylene sensitivity and response magnitude. The preharvest application of 1-MCP could potentially be used to accelerate pitahaya fruit ripening. The preharvest application of 200 μgL-1 1-MCP resulted in smaller changes in color and titratable acidity vs. control. Total soluble solids and pH were not significantly affected by the treatments. The postharvest application of 1-MCP maintained the texture of fruits by three additional days when compared to the untreated control.

The use of edible coatings on fresh and processed food products as a means of extending shelf life by preventing or delaying spoilage, providing a partial barrier to moisture, oxygen, and carbon dioxide, improving the mechanical handling properties, and even as carriers of many functional ingredients, is rapidly growing. Edible coatings can be applied by different methods such as panning, fluidized bed, dipping, and spraying. This review presents and discusses some aspects of the application of edible coatings on food products using spraying, which is the most commonly used technique for applying food coatings and to obtain uniform layers.
Navel oranges were subjected to high-temperature forced-air (HTFA) treatment to evaluate the effect on quality and sensory attributes as well as flavor volatiles of a treatment protocol designed to disinfest citrus of Anastrepha spp. fruit flies. The treatment consisted of heating the fruit to a core temperature of 44 °C and then holding it there for 100 min, after which the fruit were placed into storage for 4 weeks. The fruit were removed from storage and evaluated for surface injury, soluble solids concentration (SSC), titratable acidity (TA), and then judged for sensory characteristics by a semiexpert panel. In a separate experiment, fruit were removed at 30-min intervals from the treatment chamber and sensory quality as well as flavor volatiles determined to obtain an estimate of when the flavor changes occurred. It was found that the HTFA treatment caused a significant loss in flavor quality that was most closely linked to a loss in the fresh flavor of the fruit. The HTFA-treated fruit were also determined by panelists to be less sweet, although the SSC/TA ratio was increased by treatment. Neither storage nor waxing after treatment appeared to alter the HTFA effect, although waxing before treatment greatly enhanced the negative effect on flavor. Flavor began to be significantly affected during the final 30 min of treatment. The flavor changes occurred at the same time as large increases in the amount of four esters, two of which were present in concentrations exceeding aroma thresholds and are likely involved in the loss in flavor quality induced by HTFA treatment.
Fruit flesh browning (FB) is a major component of cold storage disorders that limits fresh and fresh cut fruit consumption. Using fertigation, nutrient deficiencies were imposed on ‘Grand Pearl’ nectarines (Prunus persica var. nectarina) grown in sand culture for 8 years and postharvest flesh browning was studied over 2 years. Antioxidant activity, polyphenol oxidase activity, total phenolics, and fruit FB potential were evaluated. Nutrient deficiencies did not always result in leaf or fruit tissue deficiency, indicating complex interactions among nutrients during uptake and use in the plant and its fruit. Low phosphorus and nitrogen fruit concentrations were associated with biochemical browning reactions in fruit flesh at harvest and with fruit FB during storage, signs of a shorter market life and lower consumer quality. Currently recommended leaf and fruit nutrient critical values are based only on production and do not address postharvest quality. Further research is needed to determine new recommended leaf and fruit nutrient values suitable for both production and maintaining fruit quality during storage.
The combined effect of sulfur dioxide (SO2) fumigation and different carbon dioxide (CO2)-enriched atmospheres (3% O2 + 3, 6, 12, or 24% CO2) on quality attributes, postharvest decay, phytochemical content, and antioxidant capacity of eight fresh blueberry cultivars (Vaccinium corymbosum L.) was determined. The SO2 treatments did not harm berry quality, but did significantly reduce decay incidence, especially when it was followed by storage in elevated CO2 atmospheres (>6%). However, very high CO2 atmospheres (24%) induced fruit softening and ‘off-flavors’. Botrytis and Alternaria spp. were the dominant fungal pathogens causing decay of blueberries during storage, but differences in the species of decay microorganisms were found among cultivars. Postharvest strategies that included SO2 fumigation and/or enriched CO2 atmospheres did not negatively affect phytochemical content or antioxidant activity of the fruit; however, the polyphenolic content and total antioxidant activity varied greatly among cultivars. Overall, SO2 fumigation followed by controlled atmosphere storage (3% O2 + 6 or 12% CO2) is a promising postharvest strategy for fresh blueberries to reduce decay, extend market life, and maintain high nutritional value.

There is some controversy regarding the respiratory pattern of loquat (Eriobotrya japonica [Thunb.] Lindl.). Thus in order to provide information on this aspect of loquat, fruit of 50-70 g, from cv. Golden Nugget were harvested in four stages of maturity: green (BBCH 709), color break (BBCH 801), yellow (BBCH 807), and orange (BBCH 809). The parameters evaluated in each stage were: soluble solids, titratable acidity, respiration, ethylene generation, and activity of the enzymes pectin methyl esterase (PME), peroxidase, polyphenoloxidase (PPO), polygalacturonase (PG), and cellulose, and the soluble solids:acidity ratio was calculated. The results show that ethylene concentration increased at the time of color break, which was not the case for the change in the respiratory rate. The activity of the peroxidase enzyme increased from the green stage to color break, while the enzymes PME, cellulase, and PG showed a constant reduction from the green to the orange stage, and PPO showed no change over the four stages studied. With regards to quality, from color break onwards soluble solids increased to 11.8 ºBrix and titratable acidity dropped from 0.67 to 0.28 g L-1 malic acid. Based on these results, the conclusion is that towards the end of its development loquat cv. Golden Nugget evidence enzymatic and ethylene behavior similar to that of climacteric fruits.

El objetivo del presente trabajo fue determinar el efecto del almacenamiento en Atmósferas Controladas (AC) sobre la calidad poscosecha y el contenido nutricional de tomate (Solanum lycopersicum L.) "Imperial". Frutos de tomate en estado de madurez comercial fueron almacenados en refrigeración (aire ambiental, AIRE) o en AC (4 kPa O2 + 96 kPa N2) a 12 °C por 21 días antes de ser transferidos a un almacenamiento en aire a 23 °C por 12 días. Se retiraron tres frutas por cada tratamiento después de cero, cuatro, ocho y 12 días de maduración a 23 °C para evaluar el color externo, la firmeza, el contenido de sólidos solubles, las velocidades de producción de CO2 y etileno y el contenido de ácido ascórbico, β-caroteno y licopeno. El almacenamiento en AC disminuyó la pérdida de firmeza y la degradación de ácido ascórbico. También disminuyó la producción de CO2 y etileno y la síntesis de β-caroteno y licopeno; además, retrasó el desarrollo del color rojo y la maduración. Los resultados indican que el almacenamiento de tomate bajo condiciones de AC prolonga la vida de anaquel y el periodo de comercialización comparado con el sistema tradicional de refrigeración.

El manejo poscosecha de camote es importante debido a que las pérdidas pueden llegar a 100% del cultivo por problemas de daño mecánico, pudrición y deshidratación. Con el manejo correcto y condiciones de transporte y almacenamiento adecuadas, se puede almacenar el camote por 4 a 8 semanas. El problema principal para el productor son los niveles de rechazo del camote en la empacadora por daño por insectos de suelo, que puede llegar hasta el 100% de cultivo, si los controles durante la producción no son adecuados.
A considerable amount of tomato is damaged after harvest every year (20-30% annually) which has impact on the total vegetable production in Bangladesh. Gamma irradiation doses of 250, 500 and 750 Gray (Gy) were analyzed compared to those of unirradiated ones on 1st, 8th and 13th day of irradiation stored at 4, 12 and 25°C to observe whether it could combat the loss. Radiation did not affect colour of tomato and it did not differ significantly with dose as well. However, storage time had effect on colour at 12 and 25°C at some aspect whereas no effect at 4°C in both irradiated and unirradiated tomatoes. Significant firmness was lost in irradiated tomatoes stored for 13 days at 4°C while no such significant differences in firmness was observed at 12 and 25°C. Immediate firmness loss was observed in irradiated tomatoes stored at 25°C and firmness decreased more in 500 and 750 Gy treated tomatoes than 250 Gy treated ones at 4 and 25°C whereas no such difference was observed between irradiation doses at 12°C. Radiation had no effect on percent sugar. Highest percentage of tomato loss was found at 25°C in both irradiated and unirradiated tomatoes but 750 Gy treated tomatoes showed promising result at all storage temperatures. Considering firmness loss,Total Soluble Solid (TSS) (%sugar) and damage during storage, a dose of 750 Gy and storage temperature 12°C stood out as ideal combination for BARI Hybrid-3 tomato in Bangladesh to combat annual post harvest loss.
Pyrimethanil (PYR) has recently been approved for postharvest use on citrus fruit to control green mold, caused by Penicillium digitatum. The EC50 of PYR to inhibit germination of P. digitatum spores was 0.2–0.4 mg/L and was similar from pH 4 to 7. Green mold on citrus fruit was reduced more than 90% by PYR at 500 mg/L or higher applied by immersing for 30 s or drenching the fruit, while its application in wax over rotating brushes at 1000 or 2000 mg/L reduced green mold about 65%. Control of sporulation by PYR in aqueous solutions was better than the same concentration applied in wax, but it was inferior to imazalil. An imazalil-resistant P. digitatum isolate was controlled by PYR. The addition of sodium bicarbonate improved PYR performance.
PYR was not compatible with chlorine. An increase in the temperature of the PYR solution slightly but significantly improved its effectiveness to control green mold, although its residues on fruit were greatly increased by heat; they approximately doubled for each 5 °C increase in solution temperature above 30 °C. PYR was very effective when applied up to 24 h after inoculation, but much less effective when itwas applied before inoculation. PYR effectively controls green mold and can be useful to control isolates of P. digitatum resistant to other fungicides.

The ‘Empire’ is a major apple variety in the northeastern United States, especially in New York, that is grown for both domestic and export markets. The cultivar is also favored for its fresh cut slice quality because of maintenance of texture and slow browning. Market demand for ‘Empire’ apples is high and the industry would like to store the fruit for at least 10 months. However, a number of physiological disorders limit the controlled atmosphere (CA) storage life for this variety. The main ones are external CO2 injury, firm flesh browning (chilling injury), soft flesh browning (senescent breakdown), and core browning.
External CO2 injury is a serious problem, but we now know that this injury can be prevented by treatment of fruit with diphenylamine (DPA), an antioxidant used for control of superficial scald, or by use of low CO2 levels in CA, especially during the earlier storage periods (Razafimbelo et al., 2006; Watkins and Nock, 2007).
Purpose of the review: Diverse means to control decay and spoilage of table grapes during storage have been described in numerous research papers that were published recently. The purpose of this review is to describe some of the major publications in this field and to point out on their advantages and shortcoming.
Main findings: Studies pertaining to the control of postharvest decay of table grapes and prevention of quality losses were categorised according to the general approach used (dry, wet, physical). Of the “dry” treatments, active modified atmosphere packaging (MAP), which includes volatiles for continuous control of decay, seems to be an attractive approach. Of the methods that can be directly integrated into current commercial practices, biofumigation with the fungus Muscodor albus warrants further assessment. Wet treatments have some inherent disadvantages because they require additional handling of the grapes. However, postharvest treatments with disinfectants such as ethanol can effectively control decay during cold storage of medium duration. These wet treatments can be useful to remove visible deposits of soil and pesticides, to prolong the storage life of organic grapes, and to disinfect and retard the decay of “ready-to-eat” grape products. Advantages of ethanol are that its efficacy can be enhanced by heat, sorbates, or MAP, and that it dries faster than other wet treatments.
Directions for future research: There are specific issues to be addressed for every technology to be considered. However, many of the studies published on the control of spoilage of table grapes report results using specific cultivars, storage of limited duration, or small scale experiments. Any technology further to be considered must be confirmed in large-scale, semi-commercial experiments to reveal potential pitfalls and limitations and to evaluate all aspects of fruit quality. In addition, a cost analysis of each technology is essential.
Common food additives (sodium bicarbonate (SB), sodium carbonate (SC), and potassium sorbate (PS)) were compared to the fungicide fludioxonil for the control of gray mold on California-grown ‘Wonderful’ pomegranates artificially inoculated with Botrytis cinerea and stored at 7.2 °C in either air or controlled atmosphere (CA, 5 kPa O2 +15 kPa CO2) conditions. Fludioxonil was superior to other treatments. PS was the most effective additive. Synergistic effects between antifungal treatments and CA storage were observed. After 15 weeks of storage at 7.2 °C, the combination of PS treatment (3 min dip in 3% solution at 21 °C) and CA storage was as effective as the combination of heated fludioxonil (30 s dip in 0.6 g L−1 of active ingredient at 49 °C) and air storage. Mixtures of PS with SB or SC did not improve the efficacy of either treatment alone. In tests conducted in commercial facilities, decay development and external and internal fruit quality were assessed on naturally infected pomegranates stored in either air or CA after application of a selected postharvest antifungal combined treatment (CTrt) integrating PS, SB + chlorine, and fludioxonil. CTrt was effective in controlling natural gray mold after 6 weeks of storage at 8.9 °C, but lacked persistence and it was not effective after 14 weeks. CA storage greatly enhanced decay control ability of CTrt. Skin red color was better maintained in CA-stored than in air-stored fruit. Juice color and properties (SSC, TA, and pH) were not practically affected by either postharvest treatment or storage condition. The integration of PS treatments with CA storage could provide an alternative to synthetic fungicides for the management of pomegranate postharvest decay.
Potassium sorbate (KS), a common food preservative, was evaluated to control postharvest decay of citrus fruit. Significant advantages of KS over the commonly used sodium bicarbonate, which similarly improved fungicide performance, are the relatively low salt concentration of KS, the absence of sodium, and its lower pH, so disposal of used KS solutions would raise fewer regulatory issues. The influence of KS concentration and pH (pH 4–9) on the germination of spores of Penicillium digitatum was determined alone or in combination with four postharvest citrus fungicides, imazalil (IMZ), thiabendazole (TBZ), pyrimethanil, and fludioxonil. The EC95 concentrations of KS to inhibit spore germination were lowest from pH 4–6. To control green mold on inoculated fruit, KS was compatible with these fungicides and consistently improved their performance. KS alone or fungicide–KS solutions were more effective when heated. The combination of KS and sodium bicarbonate was only moderately better than either alone. Green mold caused by an isolate of P. digitatum resistant to IMZ and TBZ was effectively controlled when KS was added to a heated IMZ or TBZ solutions. Heat, but not KS, increased residues of all of the fungicides in oranges. Sour rot, caused by Geotrichum citri-aurantii, was reduced from 94.5% among control lemons, to 49.1 and 47.2%, respectively, by 30 s immersion in KS or sodium bicarbonate at 1% (wt/vol) at 25 °C, and to 37.0 and 15.7%, respectively, when these solutions were at 50 °C.
Purpose of review: This article reviews research based on the evaluation of postharvest control methods alternative to conventional chemical fungicides for the control of citrus green and blue moulds, caused by the pathogens Penicillium digitatum and P. italicum, respectively. Emphasis is given to advances developed during the last few years. Potential benefits, disadvantages and commercial feasibility of the application of these methods are discussed.
Findings: Substantial progress has been accomplished in selecting and characterising new effective physical, chemical and biological control methods. However, their widespread commercial implementation relies, in general, on the integration of different treatments of the same or different nature in a multifaceted approach. For satisfactory penicillium decay control, this postharvest approach should be part of an integrated disease management (IDM) programme in which preharvest and harvest factors are also considered.
Limitations: The lack of either curative or preventive activity, low persistence, high variability, inconsistency or excessive specificity are general limitations associated with the use of alternatives to synthetic fungicides as stand-alone treatments. Furthermore, the risk of adverse effects on fruit quality, technological problems for cost-effective application, or the availability of new conventional fungicides for traditional markets are additional reasons that may hinder the broad commercial use of such treatments.
Directions for future research: As we learn more about the fundamental basis underlying host-pathogen interactions and how they are influenced by direct or indirect protective effects of existing or new single alternative treatments, more effective methods of applying and combining complementary approaches for additive or synergistic effects will emerge. Research should provide appropriate tools to tailor the application of these nonpolluting postharvest control systems and, further, the complete IDM strategy for each specific situation (ie, citrus species and cultivar, climatic and seasonal conditions, destination market, etc).
To evaluate potential alternatives to conventional fungicides to control decay, more than 20 food additives and generally regarded as safe compounds were tested at three concentrations in in vivo primary screenings with several cultivars of California peaches, nectarines, and plums that had been artificially inoculated with seven major postharvest pathogens: Monilinia fructicola, Botrytis cinerea, Geotrichum candidum, Alternaria alternata, Penicillium expansum, Mucor piriformis, and Rhizopus stolonifer. Overall, the best compounds were 200 mM potassium sorbate (PS), 200 mM sodium benzoate (SB), 200 mM sodium sorbate, 100 mM 2-deoxy-D-glucose, 400 mM sodium carbonate, and 250 mM potassium carbonate. Sodium and ammonium molybdates, acid lactic, and hydrogen peroxide were somewhat effective but were phytotoxic to fruit skin tissues. However, the best compounds lacked effectiveness and persistence when tested against brown rot in small-scale trials of 60-s dips in aqueous solutions at ambient temperatures; PS and SB reduced brown rot incidence by less than 40%. Rinsing treated fruit with tap water reduced the efficacy of the compounds by up to 30%. In contrast, heating the solutions to 55 or 60°C significantly increased treatment efficacy. Brown rot incidence and severity were reduced by 35 and 25%, respectively, on PS-treated peaches after 7 days of incubation at 20°C. However, treatment efficacy was not superior to that with water alone at these temperatures. In semicommercial trials, mixtures of fludioxonil with PS, SB, or 2-deoxy-D-glucose applied as fruit coatings on a packing line were not synergistic in their effect on brown rot, gray mold, and sour rot.
Frutos de aguacate 'Hass' fueon tratados térmicamente (38ºC y 40ºC por 360 min y 120 min respectivamente) y almacenados a 4ºC por 42 dias. La mayor calidad externa fue por el tratado a 40ºC. En calidad interna, no observándose diferencia entre control y tratados. Los frutos que desarrollaron mayor daño por frío externo fueron los tratados a 40ºC, seguidos por los controles y finalmente tratados a 38ºC. En la evaluación de daño por frío interno no se observaron diferencia entre los lotes. No existió diferencia entre los parámetros de color evaluados (a*, b*, L*, hº y C*) entre control y tratados. La pérdida de peso no demostró diferencia entre control y tratamientos. La firmeza del control fue la menor (15.93N), seguido por el tratamiento a 40ºC (41.69N) y el tratamiento a 38ºC (61.10N). Los triacilglicéridos evaluados (trilinoleina, 1,2-Dilinolein-3-Oleil-glicerol, 1,2-Dioleil-3-Esterail-glicerol), demostraron que el control presentó un comportamiento similar en todos los triacilglicérios. Los tratamientos incrementaron su concentración una vez finalizado el tratamiento térmico y posteriormente disminuye. El tratamiento a 38ºC mostró un incremento en 4 de los triacilglicéridos, no así el tratamiento a 40ºC.
Phytosanitary treatments are used to disinfest agricultural commodities of quarantine pests so that the commodities can be shipped out of quarantined areas. Ionizing irradiation is a promising phytosanitary treatment that is increasing in use worldwide. Almost 19000 metric tons of sweet potatoes and several fruits plus a small amount of curry leaf are irradiated each year in 6 countries, including the United States, to control a number of plant quarantine pests. Advantages over other treatments include tolerance by most fresh commodities, ability to treat in the final packaging and in pallet loads, and absence of pesticide residues. Disadvantages include lack of acceptance by the organic food industries and logistical bottlenecks resulting from current limited availability of the technology. A regulatory disadvantage is lack of an independent verification of treatment efficacy because pests may be found alive during commodity inspection, although they will not complete development or reproduce. For phytosanitary treatments besides irradiation, the pests die shortly after the treatment is concluded. This disadvantage does not hamper its use by industry, but rather makes the treatment more difficult to develop and regulate. Challenges to increase the use of phytosanitary irradiation (PI) are cost, because commercial use has not yet reached an optimum economy of scale, lack of facilities, because of their cost and current inability to feasibly locate them in packing facilities, lack of approved treatments for some quarantine pests, and concern about the process by key decision makers, such as packers, shippers, and retailers. Methods for overcoming these challenges are discussed.
